isolation and identification of a sulfide/sulfoxide monooxygenase gene from a newly isolated rhodococcus sp. strain fmf

Authors
abstract

rhodococcus fmf is a gram-positive bacterium isolated for the first time from soil samples of tabriz refinery in iran. this microorganism is able to catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the destruction of carbon-carbon bonds. three structural genes, dsza, dszb, and dszc have been characterized and shown to be responsible for this phenotype. in this work, dszc gene from rhodococcus fmf was identified and then isolated by pcr technique using the nucleotide sequence of dszc gene from rhodococcus erythropolis igts8 to design required primers. after amplification, the purified pcr product was cloned into plasmid vector ptz57r/t. further analysis by mapping and sequencing showed the identity of 689 nucleotides from this gene compared to the dszc gene from rhodococcus sp. strain igts8 as a key microorganism in biodesulfurization field.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Isolation and Identification of a Sulfide/Sulfoxide Monooxygenase Gene from a Newly Isolated Rhodococcus Sp. Strain FMF

Rhodococcus FMF is a gram-positive bacterium isolated for the first time from soil samples of Tabriz refinery in Iran. This microorganism is able to catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the destruction of carbon-carbon bonds. Three structural genes, dszA, dszB, and dszC have been characterized and shown to be responsible for this phenotype. In this work,...

full text

Isolation and Partial Characterization of a Bacterial Thermostable Polymethyl Galacturonase from a Newly Isolated Bacillus sp. strain BR1390

Background: Pectinases are pectin degrading class of enzymes including polygalacturonase (PG), polymethyl galacturonase (PMG), pectate lyase (PEL), and pectin esterase (PE) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices. Objectives: A highly methylated pectin degrading bacterium from soil covered with fruit wa...

full text

Isolation of Rhodococcus sp. strain ECU0066, a new sulfide monooxygenase-producing strain for asymmetric sulfoxidation.

A new and efficient sulfide monooxygenase-producing strain, ECU0066, was isolated and identified as a Rhodococcus sp. that could transform phenylmethyl sulfide (PMS) to (S)-sulfoxide with 99% enantiomeric excess via two steps of enantioselective oxidations. Its enzyme activity could be effectively induced by adding PMS or phenylmethyl sulfoxide (PMSO) directly to a rich medium at the early log ...

full text

isolation and partial characterization of a bacterial thermostable polymethyl galacturonase from a newly isolated bacillus sp. strain br1390

background: pectinases are pectin degrading class of enzymes including polygalacturonase (pg), polymethyl galacturonase (pmg), pectate lyase (pel), and pectin esterase (pe) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices.objectives: a highly methylated pectin degrading bacterium from soil covered with fruit was...

full text

Isolation and identification of bioactive compound producing Rhodococcus spp. isolated from soil samples

The genus Rhodococcus is gram positive bacterium which was classified into the family of Nocardiaceae.  The organism can be isolates from different sources, with capability to degrade diverse types of pollutant. Nowadays, scientists reported a few secondary metabolites which are produced by Rhodococci. Hence, the present study tried to isolate Rhodococci from different soil samples with the cap...

full text

My Resources

Save resource for easier access later


Journal title:
journal of sciences islamic republic of iran

جلد ۱۵، شماره ۲، صفحات ۰-۰

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023